The nature of copper in pig plasma benzylamine oxidase
نویسندگان
چکیده
منابع مشابه
Active-sitve titration of pig plasma benzylamine oxidase with phenylhydrazine.
Pig plasma benzylamine oxidase is a protein containing cupric copper and pyridoxal phosphate. The pyridoxal phosphate is stably linked to the enzyme. Discrepancies in the numbers of active sites per molecule of enzyme are reported in the literature. This paper shows that the fully active pure enzyme contains 3 mol of pyridoxal phosphate per mol, whereas enzymes with a lower specific activity ar...
متن کاملProperties of cupric ions in benzylamine oxidase from pig plasma as studied by magnetic-resonance and kinetic methods.
Benzylamine oxidase from pig plasma has been studied by a variety of chemical and physical techniques. 1. Analytical ultracentrifugation, gel electrophoresis and isoelectric-focusing studies suggest that the enzyme is composed of two subunits with closely similar primary structures. 2. E.s.r. and n.m.r. measurements show that the enzyme contains two well-separated (greater than 0.6 nm) Cu2+ ion...
متن کاملSpatial relationship between the copper and carbonyl cofactors in the active site of pig plasma amine oxidase.
The ortho-, meta-, and para-trifluoromethylphenylhydrazine inhibitors of porcine plasma amine oxidase were synthesized. Titrations of plasma amine oxidase with these inhibitors demonstrated that 1 mol of trifluoromethylphenylhydrazine completely and irreversibly modifies 1 mol of enzyme by covalently binding to the active carbonyl cofactor. NMR relaxation measurements on the fluorine nuclei wer...
متن کاملStudies on the active site of pig plasma amine oxidase.
Amine oxidase from pig plasma (PPAO) has two bound Cu2+ ions and at least one pyrroloquinoline quinone (PQQ) moiety as cofactors. It is shown that recovery of activity by copper-depleted PPAO is linear with respect to added Cu2+ ions. Recovery of e.s.r. and optical spectral characteristics of active-site copper parallel the recovery of catalytic activity. These results are consistent with both ...
متن کاملMonoamine Oxidase. Iv. Nature of the Second Prosthetic Group of Plasma Monoamine Oxidase.
Previous papers in this series have described the crystallization and preliminary characterization of beef plasma monoamine oxidase (1) as well as the fact that cupric copper is one of the prosthetic groups of this enzyme (2). In the present paper, studies related to the second prosthetic groups of this enzyme are presented and the results discussed. A preliminary report has already been publis...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1968
ISSN: 0306-3283
DOI: 10.1042/bj1060575